Detaches Adherent Cells in Minutes Dissociates Tissues for Primary Cell Culture Gentle Cell Detachment for Maximum Cell Viability Highest Plating Efficiency Cost Effective and Ready to Use Cell Detachment CELL DETACHMENT: Human MG63 fibrosarcoma cells cultured on tissue culture treated dishes in DMEM + 10% FBS were treated with ACCUTASE. Treatment results in rapid cell detachment, a single cell suspension, and high viability. ACCUTASE is gentle on cells; viability was 97 ± 3% even after 45 minutes in ACCUTASE. Typical Accutase Data Sheet:

has been developed to meet the most demanding needs for gentle and effective detachment of adherent cells. has been proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. is recommended for use with SmartPlastic* adhesion cell culture plasticware. gently and rapidly dissociates tissues for cell isolation and propagation. combines protease and collagenolytic activities which maximizes its versatility for cell detachment and tissue dissociation. does not contain mammalian or bacterial-derived products. is available in a ready-to-use formulation, quality controlled for in vitro cell culture applications.

Testing: Accutase has been extensively tested in research and biopharmaceutical laboratories.

Cell Lines: Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Application: Accutase performs exceptionally well in detaching cells for: analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Formulation: Accutase has both protease and collagenolytic activity. Each lot of Accutase is individually formulated to meet quality control standards for effective cell detachment. Accutase is supplied in Dulbecco's PBS containing O.5mM EDTA·4Na and phenol red.

Availability: Accutase is available in ready-to-use 100ml bottles.